Journal: Cell & Bioscience
Article Title: PINK1 functions in mitophagy and mitochondrial homeostasis during mice oocyte maturation
doi: 10.1186/s13578-025-01460-4
Figure Lengend Snippet: Pink1 overexpression induces mitophagy in murine oocytes. ( A ) Representative images of LC3 staining in oocytes after Pink1 -OE (scale bar = 20 μm). ( B ) The percentage of autophagy between control and Pink1 -OE oocytes (9.6 ± 1.60%, [ n = 47] vs. 31.7 ± 2.24%, [ n = 36], respectively). *Significantly different ( p < 0.05). ( C ) The effects of Pink1 -OE on LC3 protein levels, as detected by Western blotting. Proteins from 400 oocytes were loaded for each sample. ( D ) Relative intensity results for the protein expression of LC3 in control and Pink1 -OE oocytes. Proteins from 300 oocytes were loaded for each sample. ( E ) Statistical analysis of the mtDNA copy number in single oocytes from control and Pink1 -OE (88175.2 ± 5645.62 [ n = 27] vs. 61280 ± 9338.36 [ n = 35], respectively)
Article Snippet: Rabbit monoclonal anti-γH2AX antibody (Cat#: ab81299), rabbit monoclonal anti-RAB8A antibody (Cat#: ab188574), mouse monoclonal anti-Myc antibody (Cat#: ab18185) were purchased from Abcam (Cambridge, UK); rabbit polyclonal anti-PINK1 antibody (Cat#: PA5-85243) was obtained from Thermo Fisher Scientific (Thermo Fisher Scientific, USA); mouse monoclonal- FITC-conjugated anti-α-Tubulin antibody (Cat#: F2168) was bought from Sigma (St. Louis, MO, USA); rabbit polyclonal antibodies anti-LC3 antibody (Cat#: 14600-1-AP), mouse-Monoclonal antibody (Cat#: 60004-1-Ig), rabbit polyclonal antibody anti-α-Tubulin antibody (Cat#: 11224-1-AP) were purchased from Proteintech (Wuhan, China); mouse monoclonal antibody anti-PRKN antibody (Cat#: sc-32282) was obtained from Santa Cruz Biotechnologies (Santa Cruz, CA).
Techniques: Over Expression, Staining, Control, Western Blot, Expressing
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![Pink1 overexpression induces mitophagy in murine oocytes. ( A ) Representative images of <t>LC3</t> staining in oocytes after Pink1 -OE (scale bar = 20 μm). ( B ) The percentage of autophagy between control and Pink1 -OE oocytes (9.6 ± 1.60%, [ n = 47] vs. 31.7 ± 2.24%, [ n = 36], respectively). *Significantly different ( p < 0.05). ( C ) The effects of Pink1 -OE on LC3 protein levels, as detected by Western blotting. Proteins from 400 oocytes were loaded for each sample. ( D ) Relative intensity results for the protein expression of LC3 in control and Pink1 -OE oocytes. Proteins from 300 oocytes were loaded for each sample. ( E ) Statistical analysis of the mtDNA copy number in single oocytes from control and Pink1 -OE (88175.2 ± 5645.62 [ n = 27] vs. 61280 ± 9338.36 [ n = 35], respectively)](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5932/pmc12465932/pmc12465932__13578_2025_1460_Fig7_HTML.jpg)